Contact: Dr. Emila Panda (emila@iitgn.ac.in)

Model

Multimode 8(Bruker)

Features

● Contact/Tapping mode, phase imaging.
● Magnetic force microscopy.
● Conducting atomic force microscopy.
● Scanning tunnelling Microscopy.

It can be useful for:

● Topographic imaging, Phase modulation, Magnetic domain, Tunnelling current
● Electrostatic force charge distribution.
● Accurate measurement of film quality and particle size distribution.
● Measurement of Nano-scale surface roughness.
● High-resolution imaging of protein/peptide aggregate.
● High-resolution imaging of chemical scaffolds.

Contact: Dr.Sameer V. Dalvi (sameervd@iitgn.ac.in)

Model

JSM7600F (Jeol)

Features

● The microscope is equipped with two modes of operation: secondary and back-scattered electron mode.
● Energy dispersive X-ray spectroscopy (EDX – Oxford INCA Energy 250 EDS) is available for elemental analysis.
● Resolution : 1.0 nm (15 kV), 1.5 nm (1 kV).
● Magnification : Minimum 25x to maximum 1,000,000x.

It can be useful for:

● Estimation of particle morphology, particle size and size distribution.
● Elemental analysis using EDX mode.
● Estimation of morphology of micro-crack & fracture.
● Estimation of thickness of Thin-film & Oxide layer.

Contact : Dr. Iti Gupta (iti@iitgn.ac.in)

Model

Synapt G2S (Waters)

Features

● PDA Detector
● Q- TOF Detector
● Reaction monitoring
● Crude reaction mixture MS
● Stable isotope labelling
● Selective detector for GC/HPLC

It can be useful for:

● Stability studies
● Quick product identification (TLC spot)
● Confirmation of elemental composition
● Much more precise then electron absorption spectroscopy
● Molecular weight information about each chromatographic peak

Contact: Dr. Sharad Gupta (sharad@iitgn.ac.in)

Model

Autoflex Speed (Bruker)

Features

● 2 kHz smart beam-II laser technology enables ultra-high data acquisition speed.
● Laser focus diameters down to 10 µm for high spatial resolution imaging without pixel overlap.
● Wide mass range resolving power up to 26,000.
● Flash Detector combined with new 4 GHz digitizer provides 2 ppm mass accuracy for the highest confidence.
● Self-cleaning LASER source.

It can be useful for:

● Proteomics research, Bio-marker discovery.
● Analysis of biotherapeutics, Bio-assay development, and metabolite distribution.
● Intact protein sequencing

Contact : Dr. Abhijit Mishra (amishra@iitgn.ac.in)

Model

TCS SP8 (Leica)

Features

● 120nm lateral resolution
● Imaging of spectrally close fluorophores with 30% more efficiency
● The AOBS (Acousto-Optical Beam Splitter) is a completely transparent, active TeO2 crystal, which offers maximum photon efficiency
● Scan at frequencies of up to 12 kHz, resulting in about 40 fps (512 x 512 pixel) or 428 fps (512 x 16 pixel).

It can be useful for:

● Live cell imagining
● FRET assays
● Expression analysis using fluorescent probes

Contact: Dr. Chinmay Ghoroi (chinmayg@iitgn.ac.in)

Model

D8 DISCOVER (Bruker)

Features

● Powder XRD/ Thin-Film XRD.
● Small Angle XRD for Biological sample.
● High-temperature XRD (Range: Room Temperature to 1200 Degree Celsius).

It can be useful for:

● Lattice parameters, phase identity and phase purity.
● Crystallinity, crystal structure, and percent phase composition.

Contact: Dr. Vijay Thiruvenkatam (vijay@iitgn.ac.in)

Model

D8 QUEST (Bruker)

Features

● New PHOTON II detector with CPAD technology
● The largest monolithic active area of any pixel array
● Excellent sensitivity over a wide energy range (Cu Kα to In Kα radiation)
● Single-photon sensitivity
● No charge sharing effects, zero counting losses
● Air-cooled
● Sealed tube X-ray sources
● Mo or Cu radiation
● IµS 3.0 microfocus X-ray source
● Twice the intensity of conventional microfocus sources

It can be useful for:

● X-RAY crystallography of proteins
● Structural evaluation of compounds
● Lattice information

Contact: Dr. Sriram Kanvah (sriram@iitgn.ac.in)

Model

J 815 (Jasco)

Features

● Air cooled 150W Xenon lamp, highest Signal-to-Noise ratio.
● Range of precise temperature control accessories.
● Automated titration and stopped-flow accessories.

It can be useful for:

● Characterization of secondary structure (α-helix, β-sheet).
● Detection of changes in structure upon mutagenesis.
● Detection of Changes in the confirmation of a protein upon protein: protein interaction.
● Studying conformational stability of proteins in various conditions such as pH, Temperature, denaturing agents, etc.

Contact : Dr. Chinmay Ghoroi (chinmayg@iitgn.ac.in)

Model

Nexion 2000B ICP-MS (Perkin Elmer)

Features

● A new solid-state RF generator.
● Triple Cone Interface with Quadrupole Ion Deflector
● Universal cell technology with Free running Rf plasma generator
● Built-in Radian™ Remote Monitoring Service with Syngistix for ICP-MS software

It can be useful for:

● Heavy metal detection in various samples
● Detection of metals in the blood.
● Detection of Metal-based nanoparticles in aqueous solution

Contact: Dr. Superb Misra (smisra@iitgn.ac.in)

Model

Nano ZS ( Malvern Instruments)

Features

● The sensitivity to measure small and dilute samples
● Little or no dilution required for sample preparation
● The technology to measure at up to 40%w/v of particle concentration.
● Minimum sample volume of 12μL for size measurement
● Wide range of sample suitability from nanoparticles to emulsions and macromolecules
● Automation using autotitrator to improve productivity
● Data assessment using quality report and ‘Expert advice’ system
● Use as a chromatography detector for on-line size measurement

It can be useful for:

● Particle size and size distribution
● Molecular weight
● Hydrodynamic size & Zeta potential of colloids.
● Can be used to study the stability, flocculation and polydispersity of nanoparticles
● Isoelectric points of colloidal suspensions

Contact: Dr. Rupak Banerjee (rupakb@iitgn.ac.in)

Model

Rigaku SmartLab 9KW

Features

● Rotating Anode X-ray Source with 9 KW output power
● Five axis Goniometer in vertical geometry (θ-θ mode) facilitating both out-of-plane as well as in-plane scattering geometry
● 2D Hybrid pixel semiconductor detector with a large active area, high resolution and high dynamic range (Supports 0D, 1D and 2D measurement modes)
● Automatic and programmable rotary attenuator
● Easily changeable different Optical configurations (Divergent, convergent or parallel beam) to suit various application modes
● Capable of measuring several modules related to powder, thin film and Small Angle X-ray scattering modes
● High temperature attachment (room temperature to 1100 °C) for thin films

XRD can be useful for:

● Phase identification, crystallite size and lattice parameter determination
● Stress and texture estimation
● Determination of film thickness, density, roughness and composition of thin films
● Reciprocal space maps to access wide reciprocal lattice map in a short time spans

Contact: Dr. Sharad Gupta (sharad@iitgn.ac.in)

Model

UYA 2.4Y (RAD WAG)

Features

● Electronic level indicator
● Infrared sensors for door operation
● PRINT function
● Minimum sample volume of 12μL for size measurement
● TARE function

Ultra-micro balance can be useful for:

● To measure small quantities in milligram range
● Multiple measurements in single boat

Contact: Dr. Abhay Gautam (agautam@iitgn.ac.in)

Model

FEI, Themis 60-300 with EDS detector and FEI-Ceta 4k X 4k camera

Features

● FEI Titan Themis 60-300 kV.
● X-FEG high brightness gun
● New goniometer with piezo control for fine movements in x, y and z
● Super-X windowless EDX (4 quandrant SDD EDX detection; solid angle > 0.7 srad)
● 4k x 4k CMOS-based FEI CETA camera

TEM can be useful for:

● Characterization of various materials.
● Process optimization.
● High resolution imaging of various molecules.

Contact: Dr. Sivpriya kirubakaran (priyak@iitgn.ac.in)

Model

Envision Xcite Multilable Reader

Features

● High sensitivity—detects molecular interactions down to femtomolar concentrations.
● Low background—virtually no auto fluorescence or non-specific fluorescent background from plates,
● High signal/background—S/B ratios of several hundred are reached with some assays.
● Versatility in assay design—measure enzyme activity, receptor-ligand interactions, low affinity interactions, second messenger levels, functional GPCR studies, DNA, RNA, proteins, peptides, carbohydrates, small molecules, large molecules, or binding partners of greatly disparate size.
● Low affinity to high affinity interactions.

Envision Plate reader can be useful for:

● Enzyme linked Immunosorbent assays
● Enzyme kinetics
● Biochemical Assays
● Protein small molecule interaction studies using luminescence and fluorescence

Contact: Dr. Sriram Kanvah (sriram@iitgn.ac.in)

Model

Ascend NMR-500MHz (Bruker)

Features

● Correlated Spectroscopy (COSY)
● Nuclear Overhauser effect spectroscopy (NOESY)
● Heteronuclear correlation Spectroscopy (HETCOR)
● Multi nuclei NMR
● Multi-dimensional NMR
● Low temperature NMR

NMR can be useful for:

● To analyse structure of the molecule
● Quantification and purity of the drug sample
● Detection of fractions of petroleum products
● Purity assessment